Is beta-actin a loading control?
Beta-actin, also known as a “housekeeping” protein, is usually used as a loading control , for among others, the integrity of cells, protein degradation, in PCR and Western blotting. They can be used to normalize the levels of protein detected by confirming that protein loading is the same across the gel.
What is the purpose of a loading control in Western blot?
Loading control antibodies are important controls as they indicate the equal loading of samples across all wells. Loading controls also indicate the proper transfer of proteins to the membrane during the western blotting process. Loading controls are typically proteins with high and ubiquitous expression.
Is beta-actin a positive or negative control?
For this reason, measuring beta-actin levels has served as a useful control in research experiments in order to have a baseline of protein expression to compare cell manipulations to. Lanes A: Antibody dilution 1:5,000 B: Negative control (only secondary antibody).
How do loading controls work?
Loading controls are antibodies that are used to detect proteins within samples. When western blots are used to determine the levels of protein expression in a sample, loading controls ensure that the results aren’t due to loading or protein transfer errors.
How do you determine positive and negative controls?
A negative control is a control group in an experiment that uses a treatment that isn’t expected to produce results. A positive control is a control group in an experiment that uses a treatment that is known to produce results.
How is beta actin used as a loading control?
Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static…
How is beta actin used in western blot?
Beta-actin, is usually used as a loading control for Western Blot to normalize the levels of protein detected by confirming that protein loading is the same across the gel. OriGene offers high quality quality of Anti-beta actin mouse mAb (Loading control) for Western Blot.
What is the role of beta actin in muscle cells?
Beta-Actin Protein. Beta-actin is an isform of actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. There are six different but highly conserved actin isoforms in vertebrates. Four of these isoforms are expressed primarily in striated and smooth muscle cells,…
How are loading controls used in western blots?
Loading controls are essential for proper interpretation of western blots. They can be used to normalize the levels of protein detected by confirming that protein loading is the same across the gel. Beta-actin is an isform of actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity.